Microbiological and histopathological aspects of canine pyometra
نویسندگان
چکیده
As pyometra is recognized as one of the main causes of disease and death in the bitch the purposes of this study were to evaluate microbiological and histopathological aspects of canine pyometra and to research the virulence factors of the E. coli isolates identifying possible risks to human health. The microbiological isolation from the intrauterine contents of 100 dogs with pyometra was carried out and the virulence factors in the E. coli strains were identified using PCR method. This study also consisted of the counting of microorganisms colonies forming units in samples of intrauterine content, tests of antimicrobial susceptibility of the E. coli isolates and the histological examination of the uterus. E. coli was the most prevalent microorganism isolated (76.6%) and 120 strains (79.5%) were positive for sfa, 86 (56.9%) were positive for cnf, 87 (57.6%) were positive for pap, 52 (34.4%) were positive for hly, 51 (33.8%) were positive for iuc and 5 (3.3%) were positive for afa genes. One observed more sensitivity of E. coli to norfloxacin, polimixin B, sulphazotrin, chloranfenicol and enrofloxacin. In 42% of the samples of uterine walls where microorganisms were isolated, the sizes of the areas of the inflammatory responses corresponded to 39-56%. Virulence factors were identified in 98.0% of the strains evaluated, demonstrating a high frequency of potentially pathogenic E. coli. It must be considered that dogs are animals that are living in close proximity to man for thousands of years and have an important role in the transmission of E. coli to other animals and to man.
منابع مشابه
Canine Pyometra: An Update on Pathogenesis and Treatment Washington State University–Pullman
Despite six decades of research, the pathogenesis of pyometra is still not completely understood. Recent investigations question whether the previous concept of considering cystic endometrial hyperplasia (CEH)–pyometra as a complex is correct. The pathogenesis of CEH appears to have a strong hormonal component, whereas the etiology of pyometra might be more influenced by the bacterial component...
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Fransson, B. 2003. Systemic inflammatory response in canine pyometra; the response to uterine bacterial infection. Doctor’s dissertation. ISSN 1401-6257, ISBN 91-576-6393-9. Research efforts have focused mainly on the hormonal aspects of canine pyometra for more than 6 decades. However, this disease is often manifested as systemic illness in response to the bacterial uterine infection. Studies ...
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عنوان ژورنال:
دوره 39 شماره
صفحات -
تاریخ انتشار 2008